Year: 2022 | Month: April | Volume 12 | Issue 2

Effect of Ovarian Types on Retrieval of Follicles and Culturable Cumulus Oocyte Complexes in Bovine

Dipannita Baishya Arundhati Bora Anubha Baruah D.J. Dutta Champak Barman Shantanu Tamuly P.M. Barua Mohua Dasgupta
DOI:10.30954/2277-940X.02.2022.12

Abstract:

The experiment was conducted for evaluation of bovine slaughter house ovary, follicles and recovery rate of cumulus oocyte-complexes (COCs) and to compare different COCs. The collected slaughter house ovaries were classified as without corpus luteum (CL-) (type-I) and with corpus luteum (CL+) (type-II). It was found that 53.13% of the ovaries collected were without CL and 46.87% were with corpus luteum. For collection of COCs, aspiration technique was performed and accordingly number of follicles collected was recorded. The mean number of follicles recovered per type-I ovary were recorded as 5.30 which was significantly higher (P<0.01) than the corresponding values 3.27 for type-II ovary. The mean recovery of cumulus oocyte complexes were recorded as 3.41 and 1.67 per type-I and type-II ovaries respectively. The follicular materials collected from both the techniques were observed under microscope to categorize the COCs as grade A (oocyte surrounded with more than 4-5 layers of cumulus cells homogenously), grade B (oocyte surrounded with 2-4 layers of cumulus cells partially), grade C (oocyte surrounded with 1-2 layers cumulus cells) and grade D (denuded) oocytes with degeneration observed both in oocyte and cumulus cells). Grade A and grade B were classified as culturable and grade C and grade D were considered as non-culturable. Ovaries having no CL contributed more numbers of culturable COCs per ovary (3.41) than that of ovaries with CL (1.67) in aspiration technique.

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